How frequent was Rh negative blood in ancient Egypt?

In 2012, the DNA of the 20th dynasty mummies of Ramesses III and another mummy believed to be Ramesses III’s son Pentawer were analyzed by Albert Zink, Yehia Z Gad and a team of researchers under Zahi Hawass, then Secretary General of the Supreme Council of Antiquities, Egypt. Genetic kinship analyses revealed identical haplotypes in both mummies. Using the Whit Athey’s haplogroup predictor, they identified the Y chromosomal haplogroup E1b1a (E-M2).

In 2017, a team led by led by researchers from the University of Tuebingen and the Max Planck Institute for the Science of Human History in Jena tested the maternal DNA (mitochondrial) of 90 mummies from Abusir el-Meleq in northern Egypt (near Cairo), which was the first reliable data using high-throughput DNA sequencing methods. Additionally, three of the mummies were also analyzed for Y-DNA. Two were assigned to West Asian J and one to haplogroup E1b1b1 both common in North Africa. The researchers cautioned that the affinities of the examined ancient Egyptian specimens may not be representative of those of all ancient Egyptians since they were from a single archaeological site. Whilst not conclusive since the few relatively older mummies only go back to the 18th-19th dynasty, the rest being from then up to late Roman period, the authors of this study said the Abusir el-Meleq mummies “closely resembled ancient and modern Near Eastern populations, especially those in the Levant.” The genetics of the mummies remained remarkably consistent within this range even as different powers—including Nubians, Greeks, and Romans—conquered the empire.” A wide range of mtDNA haplogroups were found including clades of J, U, H, HV, M, R0, R2, K, T, L, I, N, X, W. The authors of the study noted that the mummies at Abusir el-Meleq have 6–15% maternal sub-Saharan DNA while modern Egyptians have a little more sub-Saharan ancestry, 15% to 20%, suggesting some degree of influx after the end of the empire. Other genetic studies show greater levels of sub-Saharan African ancestry in modern southern Egyptian populations and anticipate that mummies from southern Egypt would show greater levels of sub-Saharan African ancestry.

In 2018, the 4000-year-old mummified head of Djehutynakht, a governor in the Middle Kingdom of the 11th or 12th dynasty, was analyzed for mitochondrial DNA. The sequence of the mummy most closely resembles a U5a lineage from sample JK2903, a much more recent 2000-year-old skeleton from the Abusir el-Meleq site in Egypt, although no direct matches to the Djehutynakht sequence have been reported.  Haplogroup U5 is also found in modern-day Berbers from the Siwa Oasis in Egypt. A 2008 article by C. Coudray, “The complex and diversified mitochondrial gene pool of Berber populations”, recorded haplogroup U5 at 16.7% for the Siwa whereas haplogroup U6 is more common in other Berber populations to the west of Egypt.

In 2020, Yehia Z Gad and other researchers of the Hawass team published results of an analysis of the maternal and paternal haplogroups of several 18th dynasty mummies, including Tutankhamun. Genetic analysis indicated the following haplogroups:

• Tutankhamun YDNA R1b / mtDNA K
• Akhenaten YDNA R1b / mtDNA K
• Amenhotep III YDNA R1b / mtDNA K
• Yuya G2a / mtDNA K
• Tiye mtDNA K
• Thuya mtDNA K

The clade of R1b was not determined. A high frequency of R1b1a2 (R-V88) (26.9%) was observed among the Berbers from the Siwa Oasis. This haplogroup reaches its highest frequency in northern Cameroon, northern Nigeria, Chad, and Niger.